UCLA MBI — SERp Server: SER Help

Help Topics: Averaging Window BLAST Seq. ID Cutoff BLAST Sequence count weight BLAST Weight BLAST eValue Cutoff Cluster Limit Cluster Sequence Snippet Cluster Sorting Email Address Entropy Cutoff Entropy Weight Gaps Size Graph Size Graphs High Entropy Residues Last Jobs Maximum Mutations Minimum Mutations Mutable Residues Protein or DNA Sequence Queue Status Result Delivery Method Sec. Str. Cutoff Sec. Str. Weight Secondary Structure Prediction Sequence Identifier Sidechain Entropy Target Residues

Minimum number of mutations to propose for a cluster. One residue change may be sufficient to enhance crystallizability hence the default for this parameter is 1. Typically two or three mutations are required to change the entropy sufficiently. However, as the introduction of each mutation reduces solubility and can also affect the fold or structure of the protein, clusters with the lower number of required mutations are preferred. This is NOT a strict limit. If possible, clusters with as few as one mutations may be proposed.

Molecular Biology Institure at the University of California, Los Angeles.